Abstract
Solid-state fermentation was carried out using mycelium powder of Aspergillus niger as substrate for the production of chitosanase of Streptomyces. Results of the experiments indicated that the optimal medium consisted of wheat bran and mycelium powder of Aspergillus niger with initial moisture content of 60% - 70%. The enzyme activity reached 41.33 U per gram dry medium after cultured for 5 days at 28°C - 30°C and an initial pH 6.5. Chitosanase was detected on the second day of incubation and had maximal activity at 5 days and decreased gradually within a 1 month period. Solid-state fermentation is maybe an economic alternative in the production.
Highlights
Chitosan is a cationic amino polysaccharide with a linear structure of b-1,4-linked D-glucosamine (Glc N) residues [1]
Waste mycelia of Aspergillus niger, which is commonly used in a citric acid industry, was used as the induction material to produce chitosanase by solid-state fermentation of Streptomyces albolongus
The moisture content of materials plays a key role in the growth of bacteria and the accumulation of products during the solid-state fermentation process
Summary
Chitosan is a cationic amino polysaccharide with a linear structure of b-1,4-linked D-glucosamine (Glc N) residues [1]. Solid state fermentation (SSF) is a traditional fermentation technology facing new challenges. It has the advantages of high production efficiency, simple process, extensive operation, less energy consumption, less waste liquid, easy product separation, etc. Using solid state fermentation technology to produce chitosanase has better economic and environmental benefits [13] [14]. Waste mycelia of Aspergillus niger, which is commonly used in a citric acid industry, was used as the induction material to produce chitosanase by solid-state fermentation of Streptomyces albolongus. The low-cost medium for chitosanase production may facilitate process optimization for the economical production of microbial chitosanase at an industrial scale
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