Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAAGTAAGCC]-Glu-OH from the nucleoprotein of Bacillus subtilis phage phi 29.

C.M Dreef-Tromp,J.H Van Boom,G.A Van Der Marel,J.C.M Van Der Maarel,H Van Den Elst

doi:10.1093/nar/20.15.4015

Solid-phase synthesis of the nucleopeptide fragment H-Asp-Ser[pAAAGTAAGCC]-Glu-OH from the nucleoprotein of Bacillus subtilis phage phi 29.

C.M Dreef-Tromp, J.H Van Boom + Show 3 more

Open Access

https://doi.org/10.1093/nar/20.15.4015

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Journal: Nucleic acids research	Publication Date: Jan 1, 1992
Citations: 32

#Phosphite Triester Approach #Triester Approach + Show 2 more

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Abstract

The naturally occurring DNA-nucleopeptide H-Asp-Ser[5'-pAAAGTAAGCC-3']-Glu-OH was prepared via a solid-phase phosphite triester approach using N-2-(tert-butyldiphenylsilyloxymethyl)benzoyl protected nucleosides. The oligonucleotide was linked via the extremely base-labile oxalyl ester anchor to the solid support.

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