Abstract
A sensitive radioimmunoassay for the detection of Brucella abortus antibody is described. The assay, performed in flexible 96-well microplates coated with Brucella abortus antigens, utilizes 125I-labeled staphylococcal protein A to detect antibody to Brucella abortus . The parameters of the assay have been analyzed using well recognized statistical methods. Least squares analysis of antigen concentration, antiserum dilution, antigen by antiserum and replicates within antigen by antiserum, estimated an r 2 of 0.98, a coefficient of variation of 3.58 and a standard deviation of 0.10. Results of regression analysis of serum dilution versus antigen concentration (ranging from 635ug/ml to 6.35 ng/ml) indicated that an antigen concentration of 6.35ug/ml was the most efficient for describing antibody variability (r 2 = 0.98, with a coefficient of variation = 3.28). Regression analysis also revealed a closer correlation between the radioimmunoassay with complement fixation test (r 2 = 0.98) than with the standard tube test (r 2 = 0.84). Detection of specific antibody assessed by radioimmunoassay was 4 to 64 fold more sensitive than the standard tube test titer and 16 to 32 fold more sensitive than the complement fixation test titer. The results described here indicate that this radioimmunoassay is very sensitive and may be capable of discriminating between false positives and false negatives, thereby, improving the diagnostic efficiency of Brucella serologic tests.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.