Solid phase peptide synthesis on Janda Jel TM resin

Abstract

A comparative synthesis of the classic difficult sequence ACP(65–74) on 2% crosslinked Janda Jel and 1% crosslinked polystyrene–divinylbenzene, (PS–DVB, Merrifield resin) by Boc/benzyl chemistry is presented. The Janda Jel batch used was of nearly double the loading of PS–DVB, yet 12.2% less of the des-Val 65 contaminant was observed in the Janda Jel synthesis. These results suggest that the increased swelling of the Janda Jel resin relative to traditional Merrifield resin afforded a higher yield of the pure peptide by permitting greater solvent-mediated disruption of secondary structure in the resin-bound peptide.