Solid Phase Peptide Synthesis by Oxidation-Reduction Condensation. Synthesis of LH–RH by Fragment Condensation on Solid Support

Abstract

Abstract Solid phase peptide synthesis by oxidation-reduction condensation was investigated. The fragment condensation on solid support was examined by employing two types of chain elongation, that from C-terminal amino acid to N-terminal amino acid (A type elongation) and that from N-terminal amino acid to C-terminal amino acid (B type elongation). The feasibility of these two approaches A and B was demonstrated in two syntheses of LH–RH (luteinizing hormone-releasing hormone). The LH–RH prepared from the two different chain elongations showed identical mobilities on tlc, exhibiting full activities of natural LH–RH (AVS 77-33 # 215-269).