Abstract
A highly rapid, sensitive, and selective method for the determination of zinc based on the reaction of zinc(II) with 5-amino-2-(phenyldiazenyl)phenol (ACDP) and/or 5-amino-2-(p-tolyldiazenyl)phenol (ATDP) and the solid phase extraction of the Zn(II)- ACDP and/or ATDP complex with Amberlite XAD-2000 was developed, in the presence of pH = 5.6 and 6.2 using ACDP and ATDP, respectively, and Triton X-114 medium. ACDP and/or ATDP reacts with zinc to form a violet complex with a molar ratio of 2:1 (ACDP and/or ATDP to zinc). This complex was enriched by the solid phase extraction with Amberlite XAD-2000. An enrichment factor of 500 was obtained by elution of the complex from the resin with a minimal amount of isopentyl alcohol (0.2 ml). In isopentyl alcohol medium, the molar absorptivity of the complex is 1.13 × 10<sup>6</sup> and 1.37 × 10<sup>6</sup> L/mol cm at 647 and 662 nm using ACDP and ATDP, respectively, against a reagent blank prepared in a similar way without zinc. Beer's law is obeyed in the range of 5.0-160 ng/ml in the measured solution. The relative standard deviation for 10 replicate samples of 50 ng/ml level is 1.26 and 1.47%, respectively. The detection and quantification limits reaches 1.5 and 4.7 ng/ml in the original samples. The presented procedure was successfully applied for determination of zinc content in real samples such as waters, food, milk, and biological samples with satisfactory results.
Highlights
Some trace heavy metals are considered to be among the vital micronutrients, and they have a wide range of biochemical functions in all living organisms [1,2,3]
Zinc is one of the significant ingredients of proteins involved in intermediary metabolism, hormone secretion pathway, and immune system, and its enzymes participate in synthesis and decomposition of proteins, lipids, carbohydrates, and nucleic acids [4,5]
The extraction was performed on a Waters Solid Phase Extraction (SPE) device, and Amberlite XAD-2000 were used
Summary
Some trace heavy metals are considered to be among the vital micronutrients, and they have a wide range of biochemical functions in all living organisms [1,2,3]. Zinc is one of the significant ingredients of proteins involved in intermediary metabolism, hormone secretion pathway, and immune system, and its enzymes participate in synthesis and decomposition of proteins, lipids, carbohydrates, and nucleic acids [4,5]. Zinc deficiency might lead to several disorders such as growth retardation, diarrhea, immunity depression, eye and skin lesions, malfunction of wound healing, and other skin diseases [6]. In order to observe the symptoms and trace element absorption, a biological sample such as blood is often analyzed. Deficiency in trace elements including deviation of zinc from its normal level in blood is used for early diagnosis of certain
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