Abstract
Solid-Phase Extraction for Photometric Determination of Rosmarinic Acid in Lemon Balm (Melissa Officinalis) Extracts The aim of this study was evaluation of the solid-phase extraction for elimination of interference compounds from lemon balm extracts aimed for photometric determination of rosmarinic acid. In experiments, evaluated conditions were as follows: composition and volume of mobile phase, ratio between volume of sample and mass of stationary phase and flow rate of mobile phase during separation. The results indicated that interfered compounds were eliminated. The lemon balm extracts should be pretreated by adsorption on normal stationary phase (silica gel) in ratio sample volume to silica gel weight 1:1 (v/w) elution by mobile phase - diethyl ether: acetic acid (9:1; v/v) - volume - 40 times of crude extract volume - with flow rate 5 ml/min. After selection of SPE conditions, the method was validated with comparison to HPLC analysis. The results suggest that this method may be useable for determination of rosmarinic acid by photometric measurement based on the complexation of Fe2+ ions with rosmarinic acid.
Highlights
Rosmarinic acid is an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid (PEREIRA et al, 2005; PETERSEN, 1997)
The final volume was measured and the concentration of rosmarinic acid was determined by high performance liquid chromatography (HPLC) and photometric method
The solid-phase extraction was demonstrated to be very efficient for elimination of interfering compounds for precise photometric determination of rosmarinic acid from lemon balm extracts
Summary
Rosmarinic acid is an ester of caffeic acid and 3,4-dihydroxyphenyllactic acid (PEREIRA et al, 2005; PETERSEN, 1997) It is commonly found in species of the Boraginaceae and Lamiaceae. Balm is very useful for nervous agitation, and for promoting sleep, and ameliorates functional gastrointestinal dysfunction (BOUSBIA et al, 2009). It is recommended as a plant juice, cream or tea infusion for nervous complaints, lower abdominal disorders, gastric complaints, hysteria and melancholia, chronic bronchial catarrh, migraine, nervous debility, toothache, earache, headache and high blood pressure and externally, for rheumatism, nerve pains and stiff necks (compress) (DASTMALCHI et al, 2007). Rosmarinic acid is responsible for many published activities, especially antimicrobial (BAIS et al, 2002), antiallergic (ITO et al, 1998; MATSUNO et al, 2002), antiviral and anti-inflammatory activity (GEORGIEV et al, 2006; HUANG et al, 2006; KAMATOU et al, 2005)
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