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Abstract
Quantitative determination of the antibiotics enrofloxacin, oxytetracycline, and trimethoprim in aquatic samples is reported. The method involves solid-phase extraction (SPE) and preconcentration of the antibiotics, separation by thin-layer chromatography (TLC) and quantification by video densitometry. TLC separation was performed on HPTLC CN F 254 s plates with H 2 C 2 O 4 -methanol as mobile phase. Spots were detected and quantified at λ = 254 nm by videodensitometry. Limits of detection ( LOD ) and limits of quantification ( LOQ ) for TLC determination were calculated. The method was evaluated for spiked water samples. Antibiotics were extracted by solid-phase extraction (SPE) on a polystyrene-divinyl-benzene (SDB) Empore extraction disk. The extraction efficiency was checked by recovery experiments.
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