Abstract
In this paper, a method using solid-phase extraction (SPE) and HPLC/ESI-MS n for the identification of the major saponins in “Danshen Dripping Pill”, a Chinese patent medicine consisting of Salvia miltiorrhizae and Panax notoginseng, is described. Through solid-phase extraction process, the saponins in “Danshen Dripping Pill” were separated from the phenolic constituents of S. miltiorrhizae and, meanwhile, efficiently concentrated. Subsequently, these saponins were characterized by HPLC/ESI-MS n analysis. Based on the studies of MS and MS 2 spectra and the comparison with reference compounds and literature data, a total of 19 saponins were identified.
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