Abstract
A rapid decline in self-renewability, viability and function, of isolated stem cells are major hurdles in developing cell based therapies. There has been an increasing interest towards identifying a support material for maintaining stem cell features of the isolated cells. Pioneering observations of the present paper, demonstrate functionally diverse potential of Solid Lipid Nanoparticles (SLNs) in deciding the fate & behavior of mouse mesenchymal stem cell. The evidences are provided to show the dual nature of the SLNs for being a scaffold for the stem cell attachment, to retain stemness, and as reagent for inducing stem cell differentiation. Scanning electron microscopic examinations together with expression analysis were used to conform to such observations. Results of the study thus suggest that Solid lipid nanoparticles can be used as a good support material when functionalized to achieve adhesive properties and as a molecular paradigm for studying the adipocytic differentiation. We envisage a new role of SLNs towards regulating stem cell character by orchestrating the structural alignment during preparation of Solid lipid nanoparticles.
Highlights
The scanning electron microscopic (SEM) images revealed their morphological characteristics which were mainly round in shape ( Figure.-1a)
The shape and surface morphology of these synthetic made solid lipid nanoparticles were studied with TEM ( Figure.-1- b & -c)
After 24 hours of incubation, the attachment of the stem cell on Solid Lipid Nanoparticles (SLNs) film was observed under phase contrast microscope
Summary
Nanotechnology brings new possibility to stem cells research and development. Measuring in nanoscale to the design, construction, and utilization of functional structures is the hallmark of the technology [1,2,3,4]. We, custom prepared the solid lipid nanoparticles to study cellular characteristics of mesenchymal stem cell line (C3H10T1⁄2).
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
