Solid lipid nanoparticles and nanostructured lipid carriers of dual functionality at emulsion interfaces. Part I: Pickering stabilisation functionality

Abstract

Solid lipid nanoparticles and nanostructured lipid carriers are two types of lipid nanoparticulate systems, that have been primarily studied for their capability to function as active carriers, and only more recently utilised in Pickering emulsion stabilisation. Unveiling the factors that impact upon the lipid particle characteristics related to their Pickering functionality could enable the development of a liquid formulation with tailored microstructure and potentially the capacity to display a two-fold performance. In part I, this work investigates how certain formulation characteristics, namely solid-to-liquid lipid mass ratio and presence of unadsorbed surfactant in the aqueous carrier phase, affect the structural properties of the lipid particles, and in turn how these influence their Pickering stabilisation capacity. The effect of the formulation parameters was assessed in terms of the wettability and physicochemical properties of the lipid particles, including particle size, crystallinity and interfacial behaviour. Lipid particles fabricated with higher liquid lipid content (70% w/w) were shown to be more hydrophilic and have lower surfactant decoration at their surface compared to particles containing lower or no liquid lipid in their crystalline matrix. The emulsion stabilisation ability through a Pickering mechanism was confirmed for all types of lipid particles using polarised microscopy. Increasing liquid lipid content and removal of excess surfactant did not compromise the particle stabilisation capacity, though emulsion droplets of larger sizes were initially acquired in the latter case. The particle-stabilised emulsions maintained their physical integrity, with particles retaining close association with the emulsion interface over a storage period of 12 weeks.