Abstract
Solid-contact ion-selective electrodes for histamine (HA) determination were fabricated and studied. Gold wire (0.5 mm diameter) was coated with poly(3,4-ethlenedioxythiophene) doped with poly(styrenesulfonate) (PEDOT:PSS) as a solid conductive layer. The polyvinyl chloride matrix embedded with 5,10,15,20-tetraphenyl(porphyrinato)iron(iii) chloride as an ionophore, 2-nitrophenyloctyl ether as a plasticizer and potassium tetrakis(p-chlorophenyl) borate as an ion exchanger was used to cover the PEDOT:PSS layer as a selective membrane. The characteristics of the HA electrodes were also investigated. The detection limit of 8.58 × 10−6 M, the fast response time of less than 5 s, the good reproducibility, the long-term stability and the selectivity in the presence of common interferences in biological fluids were satisfactory. The electrode also performed stably in the pH range of 7–8 and the temperature range of 35–41 °C. Additionally, the recovery rate of 99.7% in artificial cerebrospinal fluid showed the potential for the electrode to be used in biological applications.
Highlights
Histamine (HA) is synthesized through the oxidative decarboxylation of the amino acid histidine
In order to test the possibility of HA determination in biological applications, artificial cerebrospinal fluid was used as the background in the standard addition method
The HA selective membrane was prepared by mixing Fe(TPP)Cl as the ionophore, KTFPB as the ion exchanger and poly(vinyl chloride) (PVC) as the membrane matrix in a tube
Summary
Histamine (HA) is synthesized through the oxidative decarboxylation of the amino acid histidine. Some methods are of good sensitivity and specificity, they are restricted by their high costs, their time-consuming properties, the need for derivation and pretreatment due to the complexity of the sample matrix, and the low analytical concentrations of HA In comparison with these methods, electrochemical analyses have been rapidly developed in recent years due to their advantages of low cost, fast responses, reproducibility, reasonable sensitivity and storage stability [18,19,20]. Dibenzo-30-crown-10 shows no response to monocationic HA, which leads to the invalidation of HA sensors when the pH values of solutions are higher than 6 [31] Compared with these ionophores, metalloporphyrins bond more stably with aromatic amines than aliphatic amines. In order to test the possibility of HA determination in biological applications, artificial cerebrospinal fluid was used as the background in the standard addition method
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