Solation, Screening and Optimization for Laccase production by Fusarium spp. under submerged fermentation

Abstract

Laccases are oxidases with broad substrate specificity and ability to oxidize various phenolic and nonphenolic compounds. This study was carried out to isolate and characterizes laccase producing fungi from the environmental samples. Soil samples were collected from different locations. Suspensions of the samples (1g in 10 ml sterile physiological saline) were serially diluted, inoculated onto Czapek Dox Agar (CZA) containing antibiotic chloramphenicol and incubated for 7 days at 30 o C. The fungal isolates were characterized macroscopically and microscopically. Screening was done using Czapek-Dox agar was supplemented with 0.1% tannic acid, 0.5% Bromophenol blue, 0.05 ml in 25 ml of Methanol Guaiacol the pure culture of the single colonies from the spread plate method were inoculated on the media and incubated at room temperature for 5-6 days. After 5-6 days the plates were checked for clear transparent zone.