Solanum Nigrum polysaccharide (SNL) extract effects in transplanted tumor-bearing mice--erythrocyte membrane fluidity and blocking of functions.

Xiao-Lei Liu,Hong-Liang Yuan,Ying-Jie Liu

doi:10.7314/apjcp.2014.15.23.10469

Abstract

Solanum nigrum L. has been used in traditional Chinese medicine because of its diuretic and antipyretic effects. The present research concerned effects of crude polysaccharides isolated from Solanum nigrum L. on erythrocyte membranes of tumor-bearing S180 and H22 in mice. Fluorescence- labeled red blood cell membranes were used with DPH fluorescence spectrophotometry to examine erythrocyte membrane fluidity, and colorimetry to determine degree of erythrocyte surface membrane blocking. Extent of reaction by tumor-bearing mice with the enzyme erythrocyte membrane bubble shadow detection of red cell membrane variation in the degree of closure before and after administration. Solanum nigrum polysaccharide could significantly improve the S180 and H22 tumor-bearing mice erythrocyte membrane fluidity, compared with the control group, the difference was significant (p<0.01), SNL can significantly improve the red blood cell membrane and then S180 tumor-bearing mice sealing ability, compared with the negative control group, the difference was significant(p<0.05, p<0.01). H22 tumor-bearing mice can increase red cell membrane and then sealing ability, the difference was significant (p<0.05). Solanum nigrum polysaccharide degree of fluidity and blocking two transplanted tumors in mice restored the ability to raise the red cell membrane has a significant effect. Solanum nigrum L.-type mice transplanted tumor can affect the red blood cell membrane fluidity and re-closed, through the red cell membrane of red blood cells to enhance the immune function of the possibility of erythrocyte immunity against tumor formation garland provide experimental basis.

Highlights

Red cell membrane is a barrier to life activities of cells play a protective role, change and maintain the normal function of the cell membrane is closely related to the characteristics of the drug to act on the cells through the cell membrane must first change will inevitably affect cell membrane function internal physiological functions the change, so from the perspective of the red cell membrane function were studied very meaningful (Hongsheng et al, 2014)
Different doses of Solanum Nigrum Polysaccharide (SNL) Extract on Tumor-bearing Mice Transplanted Tumor Erythrocyte Membrane Fluidity and Blocking Degree of Functionality activities in accordance with the membrane structure and Effect of Solanum nigrum polysaccharide on erythrocyte function of the plasma membrane, or cell membrane fluidity membrane S180 and H22 blocking degree is important in maintaining normal cell physiological
The results showed that cell membrane significantly increased the role of high-dose tumor-bearing mice erythrocyte membrane fluidity than 125mg/kg blocking degree can be increased to 55.01%, normal erythrocyte membrane fluidity significantly a significant difference (p

Summary

Introduction

Red cell membrane is a barrier to life activities of cells play a protective role, change and maintain the normal function of the cell membrane is closely related to the characteristics of the drug to act on the cells through the cell membrane must first change will inevitably affect cell membrane function internal physiological functions the change, so from the perspective of the red cell membrane function were studied very meaningful (Hongsheng et al, 2014). Anti-tumor effects of red blood cells are mainly through the regulation of immune complexes include two aspects, one of the complement antigen antibody immune complex regulations, and the other is to complement antigen complex regulation Many antigens such as tumor cells can be activated directly adhered complement and red blood cells by complement receptors are adhered to carry. Solanum nigrum polysaccharide degree of fluidity and blocking two transplanted tumors in mice restored the ability to raise the red cell membrane has a significant effect. Conclusions: Solanum nigrum L.-type mice transplanted tumor can affect the red blood cell membrane fluidity and re-closed, through the red cell membrane of red blood cells to enhance the immune function of the possibility of erythrocyte immunity against tumor formation garland provide experimental basis

Methods

Results

Conclusion