Abstract
A high percentage of isolated shoot tips of Solanum etuberosum survived slow cooling at 0.3°C/min to −40°C. Below this temperature survival decreased. High survival could be obtained at −196°C by a two-step procedure of slow cooling to −40°C followed by immersion in liquid nitrogen. Dimethylsulfoxide was the cryoprotectant used in all studies. Essential for survival under these conditions was the culture in light of isolated shoot tips for a period of time (about 2 days) before addition of cryoprotectant and subsequent freezing. Under these conditions, formation of a multiple shoot mass was the predominant morphogenetic response.
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