Sol–Gel Derived Nanoporous Cerium Oxide–Titanium Oxide Platform for Glucose Sensor

Abstract

Glucose oxidase has been immobilized onto sol–gel derived nanoporous CeO2–TiO2 film deposited onto indiumtin-oxide (ITO) glass plate for construction of glucose sensor. X-ray diffraction (XRD), atomic force microscopy (AFM), Fourier transform infrared (FTIR) spectroscopy and electrochemical techniques have been used for characterization of GOx/CeO2–TiO2/ITO bioelectrode. Electrochemical response of GOx/CeO2–TiO2/ITO bioelectrode is found to be linear in the range of 0.56–22.2 mM, sensitivity of 53.48 � Am M −1 cm 2 and correlation coefficients R = 0� 987 and detection limit of 10 mg/dl with relative standard deviation of 0.00324 in lower detection range of glucose. A lower value of apparent Michaelis–Menten constant (Km� , of 1.95 mM, exhibits high affinity between the glucose and GOx immobilized on sol–gel derived nanoporous CeO2–TiO2 film. These results demonstrate that sol–gel derived nanostructured CeO2–TiO2 platform can be used for potential applications in biosensors.