Soil saprophytic fungi could be used as an important ecological indicator for land management in desert steppe

Abstract

Soil microbes are among the most complex and diverse communities on Earth, actively participating in biogeochemical cycles while responding positively to environmental changes. Saprophytic fungi are important factors in soil carbon cycle, and the response and driving factors in grassland to forestland and grassland to farmland conversion in desert steppe are still poorly understood. Therefore, we analyzed the soil microbial community structure and saprophytic fungal communities in grasslands, shrub lands, and farmlands in typical desert grassland areas of northwest China. It was found that the cultivation of desert steppe plants/crops increased the proportion of silt in soil aggregates while decreasing soil salinity and increasing soil total carbon, nitrogen, and organic matter content. Proteobacteria, Actinobacteriota and Ascomycota were the dominant phyla in desert steppe soils, with Proteobacteria, Gemmatimonadota and Mucoromycota were more responsive to soil environment changes. The co-occurrence network relationship further characterized the process of niche sharing/competition between bacteria and fungi. The complexity of the co-occurrence network was deepened by land use, and fungi occupied more of the niche in shrub planting than in desert steppe and maize planting. Network relationships further characterized the process of niche sharing/competition between bacteria and fungi, and land use deepened the complexity of the co-occurrence network, with soil fungi occupying more ecological niches in shrub cultivation than in grassland and maize cultivation. In addition, saprophytic fungi are the main functional fungi in bacterial-fungal co-occurrence networks and are influenced by soil properties. Random forest analysis further explained the importance of saprophytic fungi in response to environmental changes in desert steppes. The enrichment of soil saprophytic fungi had an obvious correlation with soil aggregate stability, soil physical properties, and soil nutrients, especially in the background of shrub forestation in desert steppes. This study further highlights afforestation as one of the ways to benefit land management in desert steppes, while emphasizing the importance of saprophytic fungi as ecosystem indicators.