Soil patch heterogeneity improves plant productivity and nutrients regulated by arbuscular mycorrhizal fungi

Abstract

AbstractThe interlacing and mosaic distribution of karst soil and rock result in the high spatial heterogeneity of the natural karst habitat. Karst plants are suffered from spatially heterogeneous microhabitats with different‐sized patches. Arbuscular mycorrhizal (AM) fungi are crucial in regulating plant productivity and nutrients in heterogeneous karst environments. However, how spatial patch heterogeneity influences plant productivity and nutrient utilization by AM fungi regulation in karst soil remains unclear. A simulated heterogeneity experiment regarding soil grid patches was conducted by planting Bidens pilosa into a microcosm of a square device. Experimental treatments included the mycorrhizal fungus treatments inoculation with (M+) or non‐inoculation with (M−) Glomus etunicatum fungus, and the spatial heterogeneity treatments involved homogeneous patches (HO), heterogeneity‐small patches (HS), and heterogeneity‐large patches (HL). The growth and nutritional traits of plants were analyzed. The results showed that AM fungus promoted the productivity and nutrient uptake of B. pilosa by substantial increases in biomass, nitrogen (N) accumulation, phosphorus (P) accumulation, and N/P ratio. The spatial heterogeneity promoted the biomass and N and P accumulations of B. pilosa when inoculating with AM fungus. The mycorrhizal colonization rate and hyphae length were greater in HS than in HO and HL under the M+ treatment, with a greater response ratio of biomass, N and P under HS conditions. These indicated that the heterogeneous small patches had a greater promotion of the biomass production and nutrients of B. pilosa with inoculation of AM fungus because extensive fungal hyphae can acquire soil resources in more distant patches outside the root zone with more economical metabolic costs compared to roots. We thus conclude that patch heterogeneity contributes to plant productivity and nutrient utilization regulated by AM fungi in karst soil, and the broader nutrient capture induced by extraradical hyphae of AM fungi may be an adaptive mechanism for karst plants to cope with heterogeneous environmental conditions.