Soil microbial community development across a 32-year coastal wetland restoration time series and the relative importance of environmental factors

Abstract

Soil microbes play key roles in ecosystem functioning through processes such as organic matter decomposition, nutrient and carbon cycling, and regulating vegetation structure and productivity. Coastal marshes are situated at the confluence of terrestrial and marine ecosystems; thus, their soils support abundant and diverse microbiota which facilitate globally important biogeochemical processes including nutrient cycling and greenhouse gas fluxes. With coastal marsh ecosystems threatened by relative sea level rise, subsidence, and development, mitigation for the loss of marshes is becoming commonplace. Despite the widespread implementation of marsh construction projects, little is known about the development and variability of microbial communities in created marshes. For this study, we tested the hypothesis that microbial community composition differs across created marshes of different ages and between created and natural marshes. We also hypothesized that the microbial community would be correlated with soil properties including soil organic carbon and nitrogen content, which were predicted to increase with marsh age. To test these hypotheses, we determined dominant microbial groups and environmental characteristics from six constructed marshes ranging in age from 0 to 32 years old, as well as an adjacent natural marsh. Our results revealed that microbial biomass estimates increased with marsh age except for the high elevation 20-year old marsh, yet each marsh contained a distinct microbial community composition. Across marshes, microbial community composition was significantly related to soil C:N ratio with an increase in total microbial abundance and actinomycetes and GM+ bacteria with an increase in soil C to N ratio. Additionally, shifts in dominant microbial groups were associated with differences in vegetation, elevation, and marsh age. The natural marsh community grouped more closely with younger rather than older marshes suggesting age is less important than environmental conditions. This study shows that microbial communities are not homogenized across restoration sites and do not necessarily converge to be similar to natural marshes over time. Local biotic and environmental conditions were correlated with microbial compositions, yet in seemingly similar conditions, microbial groups also differed. The effect of differences in microbial compositions on ecological functions are yet to be fully understood.