Soil microbial communities and extracellular enzyme activity in the New Jersey Pinelands

Abstract

We have much to learn about the roles of various groups of soil microorganisms in the decomposition of soil organic matter. Any changes in the type or amount of organic matter entering the soil, due to increasing atmospheric nitrogen (N) deposition and elevated carbon dioxide, could directly affect soil microbial community structure or the decompositional functions performed by the various microbial groups. We experimentally altered soil microbial communities using a factorial combination of trenching and in-growth bags crossed with fertilization treatments consisting of two forms of inorganic N and three N-containing organic molecules of increasing molecular weight and complexity. We tested three hypotheses: (1) Different components of soil microbial communities change in different ways following the application of fertilization treatments; (2) soil fungi decrease with increased inorganic N but increase following the application of organic molecules; and (3) activity of the extracellular enzymes peroxidase and phenol oxidase, which are important in lignin degradation, decrease following the addition of inorganic N. We found that the abundance of soil microbes and their composition (measured by lipid analysis) was significantly altered following the addition of glutamic acid, but not with inorganic N or more complex N-containing organic molecules. Lipids indicative of ectomycorrhizal fungi experienced the greatest increase in abundance. Extracellular enzyme activity, in contrast, changed very little and did not parallel changes in the structure of the soil microbial community that resulted from the isolation treatments. We conclude that small additions of N-containing organic compounds can cause changes in the structure of the soil microbial community but that community changes do not necessarily have an impact on extracellular enzyme activity.