Soil bacterial succession with different land uses along a millennial chronosequence derived from the Yangtze River flood plain

Abstract

Wetlands reclamation has been a traditional and effective practice for obtaining new land to alleviate the pressure induced by population growth. However, the evolution of soil-dwelling microorganisms along with reclamation and the potential influence of land-use patterns on them remain unclear. In this study, a soil chronosequence derived from Yangtze River sediments was established, comprising of circa 0, 60, 160, 280, 2000, and 3000 years, to examine the succession of soil bacterial communities across different land uses. Our analysis revealed obvious development in soil properties and orderly bacterial succession along reclamation gradients. Over time, reclaimed land suffered from varying degrees of abundance loss and biodiversity simplification, with dryland being the most sensitive to reclamation duration changes, whereas woodland and paddies showed slight reductions. Bacterial communities tended to shift from oligotrophs (K-strategist) to copiotrophs (r-strategist) at the phylum level as reclamation proceeded for all land use types. The relative abundance of certain bacterial functional groups associated with the carbon (C) and nitrogen (N) cycles were significantly increased, including those involved in Aerobic chemoheterotrophy, Chitinolysis, Nitrate reduction, Nitrate respiration, and Ureolysis, while other groups, such as those related to Fermentation, Methylotrophy, Nitrification, and Hydrocarbon degradation, exhibited decreased expression. Notably, prolonged reclamation can also trigger ecological issues in soil, including a continuous increase of predatory/exoparasitic bacteria in dryland and woodland, as well as a significant increase in pathogenic bacteria during the later stages in paddy fields. Overall, our study identified the impact of long-term reclamation on soil bacterial communities and functional groups, providing insight into the development of land-use-oriented ecological protection strategies.