Soil bacterial communities are more sensitive to short-term nitrogen deposition than fungal communities in subtropical Chinese fir forests

Abstract

The increasing rate of atmospheric nitrogen (N) deposition seriously affects the structure and function of ecosystems. Although soil microorganisms play a crucial role in ecosystem carbon (C) and nutrient cycling, the impact of N deposition on soil microbial communities in subtropical forest ecosystems is unclear. Here, we selected the subtropical Chinese fir (Cunninghamia lanceolata) forest as the research object and conducted simulated N deposition experiments with different levels of N deposition (0 kg N ha−1 yr−1,50 kg N ha−1 yr−1, 100 kg N ha−1 yr−1, and 200 kg N ha−1 yr−1, respectively) to explore the response of soil nutrients, soil microbial community structure, and potential ecological functions to short-term N deposition. We found that the bacterial richness Chao1 index and phylogenetic diversity PD_whole_tree decreased with the N deposition level approximately 13 % −16 % under MN and HN treatment. N deposition significantly altered the composition of soil bacterial communities (P < 0.05), but the responses of different taxa to N deposition were inconsistent at different stages of growing season. N deposition treatment significantly increased the relative abundance of Actinobacteria at both beginning of growing season (BGS) and end of growing season (EGS), decreased the relative abundances of Planctomycetes at BGS, and decreased the relative abundances of Elusimicrobia and Cyanobacteria at both BGS and EGS. Non-metric multidimensional scaling (NMDS) and Anosim (Analysis of similarities) analyses showed that the bacterial communities were clearly distinguished among treatments at both BGS and EGS. However, N deposition had a relatively small impact on fungal communities, only altering the fungal community structure at BGS. The impact of N deposition on soil microbial communities was mainly induced by the alteration in soil water content (SWC), pH, NO3–-N, and available nitrogen (AN). Both bacterial and fungal communities exhibit seasonal community dynamics, while the interaction of N deposition and season was mainly reflected in the impact on bacterial communities. Our research not only provides research ideas for the potential impact of nitrogen deposition on subtropical forest ecosystems in the short term, but also provides a theoretical basis for future scientific management and regulation of forestry, microorganisms, and sustainable management of Chinese fir plantations.