Abstract
The development of the mining industry has led to significant environmental pollution around mining sites, affecting the nitrogen (N) cycle and associated microorganisms and disrupting the structure of soil aggregates. In this study, mine soil incubation experiments were conducted under the application of two types of N fertilizer (urea (U) and ammonium chloride (AC)) and nine different fertilization patterns, and the effects of the different N fertilization patterns on soil aggregate properties and diazotrophic community structure were compared. The results showed that carbon (C), phosphorus (P) and N were mainly enriched in megaaggregates (2–4 mm) and macroaggregates (0.25–2 mm). N fertilizer addition decreased the bioavailable heavy metal (HM) content of soil aggregates to different degrees. In addition, N fertilization resulted in significantly higher activities of cellulase, invertase, amylase, urease, protease and phosphatase in microaggregates (<0.25 mm) than in mega- and macroaggregates (p < 0.05). Among the nine fertilization patterns, AC application was performed at a low frequency and high concentration. In most cases, U application resulted in a higher nifH gene abundance than AC application. Specifically, nifH gene abundance in microaggregates in the four U treatments ranged from 9.75 × 106 to 16.63 × 106 gene copies g−1 dry soil, which was significantly higher than that in the control (p < 0.05). Proteobacteria was the major diazotrophic phylum, accounting for 52.9 % to 79.1 %, 46.4 % to 80.3 % and 49.1 % to 81.2 % in mega-, macro- and microaggregates, respectively, followed by Firmicutes and Actinobacteria. The application of N fertilizers had different impacts on soil physicochemical properties, which in turn indirectly affected the abundance and community composition of soil diazotrophs. Among the aggregate properties, microbial nitrogen (MBN) was an important variable that explained the variation in diazotrophic communities (p < 0.05). Our results suggest that the addition of N fertilizer affects the physicochemical properties and the structure and functions of diazotrophic community in soil aggregates.
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