Soehngenia longivitae sp. nov., a Fermenting Bacterium Isolated from a Petroleum Reservoir in Azerbaijan, and Emended Description of the Genus Soehngenia.

Tamara N Nazina,Salimat K Bidzhieva,Denis S Grouzdev,Diyana S Sokolova,Tatyana P Tourova,Azhdar K Talybly,Andrey B Poltaraus,Alexander N Avtukh,Sofiya N Parshina

doi:10.3390/microorganisms8121967

Abstract

A methanogenic enrichment growing on a medium with methanol was obtained from a petroleum reservoir (Republic of Azerbaijan) and stored for 33 years without transfers to fresh medium. High-throughput sequencing of the V4 region of the 16S rRNA gene revealed members of the genera Desulfovibrio, Soehngenia, Thermovirga, Petrimonas, Methanosarcina, and Methanomethylovorans. A novel gram-positive, rod-shaped, anaerobic fermentative bacterium, strain 1933PT, was isolated from this enrichment and characterized. The strain grew at 13–55 °C (optimum 35 °C), with 0–3.0% (w/v) NaCl (optimum 0–2.0%) and in the pH range of 6.7–8.0 (optimum pH 7.0). The 16S rRNA gene sequence similarity, the average nucleotide identity (ANI) and in silico DNA–DNA hybridization (dDDH) values between strain 1933PT and the type strain of the most closely related species Soehngenia saccharolytica DSM 12858T were 98.5%, 70.5%, and 22.6%, respectively, and were below the threshold accepted for species demarcation. Genome-based phylogenomic analysis and physiological and biochemical characterization of the strain 1933PT (VKM B-3382T = KCTC 15984T) confirmed its affiliation to a novel species of the genus Soehngenia, for which the name Soehngenia longivitae sp. nov. is proposed. Genome analysis suggests that the new strain has potential in the degradation of proteinaceous components.

Highlights

IntroductionThe genus Soehngenia belongs to the family Tissierellaceae [1] of the order Tissierellales of the class (not validly published) ‘Tissierellia’ [2] within the phylum Firmicutes
The genus Soehngenia belongs to the family Tissierellaceae [1] of the order Tissierellales of the class ‘Tissierellia’ [2] within the phylum Firmicutes
At the time of writing, the genus Soehngenia was represented by the type strain BOR-YT (=DSM 12858T = ATCC BAA-502T) of the only published species Soehngenia saccharolytica isolated from an anaerobic sludge bed reactor treating potato starch waste [3]

Summary

Introduction

The genus Soehngenia belongs to the family Tissierellaceae [1] of the order Tissierellales of the class (not validly published) ‘Tissierellia’ [2] within the phylum Firmicutes. At the time of writing, the genus Soehngenia was represented by the type strain BOR-YT (=DSM 12858T = ATCC BAA-502T) of the only published species Soehngenia saccharolytica isolated from an anaerobic sludge bed reactor treating potato starch waste [3]. Strain DSM 12858T is a gram-positive, rod-shaped, motile, mesophilic, neutrophilic anaerobic spore-former, which ferments a wide range of carbohydrates and other carbon sources, including yeast extract, cysteine, and serine. Using 16S rRNA gene sequencing, members of the genus Soehngenia were detected in petroleum reservoirs [5–8] and in water droplets dispersed in heavy oil [9], in methanogenic crude oil- and propionate-degrading enrichments [10,11], in marine sediments contaminated with heavy metals and polycyclic aromatic hydrocarbons [12], and in cyanide-degrading enrichments [13]

Methods

Results

Conclusion