Sodium phosphate cotransporters in intestinal absorption of phosphorus in uremic rats

Abstract

Hyperphosphatemia is a common disorder in patients with chronic kidney disease (CKD). We investigate the effect of diets with distinct concentrations of P in the regulation of cotransporters NaPIIb and PiT1 in the intestine of rats. 66 male Wistar rats were used, 33 of which were submitted to nephrectomy 5/6 (Nx) and the others were used as controls (C). The animals received a standard (ST) diet (20% protein and 0.54% P) for 30 days, after they were divided into 2 sets of 3 groups in accordance with the concentration of P in the diet (0.2%↓, 0.54% or 0.9%↑) and fed for 2 days. Blood samples and 24h urine were collected and the expressions of the cotransporters were analyzed by immunofluorescence and Western blot (WB). The table summarize the results (mean ± SD). NaPIIb in ileum of Nx rats. (A) Negative control (B) ↓P; (C) ST; (D) ↑P The concentrations of Cr, P, FGF23, PTH and Albu were higher in Nx animals. Pu decreased in Nx animals and some of these parameters were modified by the concentration of dietary P. The expression of NaPIIb and PiT1 and the WB analysis are being finalized.