Abstract
Objective: to enhance the efficiency of treatment for limb ischemia-reperfusion syndrome in an experiment, by using the modified hemosorbents that have oxidative properties. Materials and methods. The investigation was conducted on 94 mongrel male dogs divided into 3 groups: 1) intact animals (n=20); 2) animals treated with hemocarboperfusion on the standard sorbent CKH-1K (n=36); 3) animals received hemocarboperfusion on sodium hypochloride-modified sorbent CKH-1K (n=38). A model of acute ischemia-reperfusion syndrome was created by the method of V. D. Pasechnikov et al. Partial oxygen tension (pO2) was determined by pin polarography. The levels of vasoactive eicosanoids were measured by enzyme immunoassay. Results. In the animals with leg ischemia syndrome, there is a significant pO2 reduction in the muscles of the hip and shin, which does not completely recover after reperfusion. Standard CKN-1K sorbent hemocarboperfusion reduces pO2 as compared with the reperfusion period while the use of modified CKH-1K hemosorbent increased pO2 in the study hind limb muscles to the level observed in intact animals. The development of ischemia and reperfusion is accompanied by the elevated levels of inflammatory mediators that have vasoconstrictive properties (thromboxane B2, endothelin-1, leukotrienes C4/D4/E4) and the lower concentration of the vasodilator prostacyclin. Standard CKN-1K sorbent hemocarboperfusion results in a further increase in the concentrations of thromboxane B2 and leukotrienes C4/D4/E4, a decrease in the concentration of endothelin-1, and an elevation of the levels of prostacyclin and prostaglandin E2. When sodium hypochlorite-modified CKN-1K sorbent hemocarboperfusion is employed, the concentrations of thromboxane B2, endothelin-1, and leukotrienes C4/D4/E4 decrease, and the level of prostacyclin increases. Conclusion. Hemocarboperfusion used in the treatment of leg ischemia-reperfusion syndrome leads to restoration of tissue oxygenation and can restore compensatory mechanisms at the tissue and metabolic levels. At this time, sodium hypochlorite-modified CKN-1K sorbent has the highest efficacy.
Highlights
Цель настоящего исследования — повышение эффективности лечения синдрома ишемии — реперфузии конечности в эксперименте с использованием модифицированных гемосорбентов с окислительными свойствами
Гемо карбоперфузия на стандартном сорбенте СКН 1К приводит к снижению рО2 по сравнению с периодом реперфузии, в то время как использование модифицированного гемосорбента СКН 1К сопровождается увеличением рО2 в иссле дуемых мышцах задней конечности до уровня интактных животных
The investigation was conducted on 94 mongrel male dogs divided into 3 groups: 1) intact animals (n=20); 2) animals treated with hemocarboperfusion on the standard sorbent CKH 1K (n=36); 3) animals received hemocarboperfusion on sodium hypochloride modified sorbent CKH 1K (n=38)
Summary
Sodium Hypochlorite Modified Hemosorbents in the Treatment of Limb Ischemia Reperfusion Syndrome: Experimental Study. У животных с синдромом ишемии конечности происходит снижение рО2 в мышцах бедра и голени не восстанавливающееся полностью после реперфузии. Гемо карбоперфузия на стандартном сорбенте СКН 1К приводит к снижению рО2 по сравнению с периодом реперфузии, в то время как использование модифицированного гемосорбента СКН 1К сопровождается увеличением рО2 в иссле дуемых мышцах задней конечности до уровня интактных животных. Hemocarboperfusion used in the treatment of leg ischemia reperfusion syndrome leads to restoration of tissue oxygenation and can restore compensatory mechanisms at the tissue and metabolic levels. At this time, sodium hypochlorite modified CKN 1K sorbent has the highest efficacy. Эти методы позволяют модели ровать внутри и вне организма естественные процессы его очищения или являются существенным к ним дополнением, что в случае нарушения детоксицирующих систем организ ма дает возможность временного их замещения [3,4,5]
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