Sodium hydroxide treatment effectively inhibits PrPCWD replication in farm soil

Hyun-Joo Sohn,Kyung-Je Park,In-Soon Roh,Hyo-Jin Kim,Hoo-Chang Park,Hae-Eun Kang

doi:10.1080/19336896.2019.1617623

Hyun-Joo Sohn, Kyung-Je Park + Show 4 more

Open Access

https://doi.org/10.1080/19336896.2019.1617623

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Journal: Prion	Publication Date: Jan 1, 2019
Citations: 9	License type: open-access

Affiliation: Animal and Plant Quarantine Agency

Abstract

ABSTRACTChronic wasting disease (CWD) agents are shed into biological samples, facilitating their horizontal transmission between cervid species. Once prions enter the environment, binding of PrPCWD by soil particles may maintain them near the soil surface, posing a challenge for decontamination. A 2 N sodium hydroxide (NaOH) or 2% sodium hypochlorite (NaClO) solution is traditionally recommended for prion decontamination of equipment and surfaces. Using protein misfolding cyclic amplification with beads and a bioassay with TgElk mice, we compared the effects of these disinfectants in CWD-contaminated soil for 1 or 16 h to those of controls of known infectious titres. Our results suggest that 2 N NaOH in a 1/5 farm soil volume provides a large decrease (>102-fold) in prion infectivity.

Highlights

Chronic wasting disease (CWD) is a prion disease affecting cervids, which is recognized in North America, Canada, Republic of Korea, Norway, and Finland [1,2,3]
Our results suggest that 2 N NaOH in a 1/5 farm soil volume provides a large decrease (>102-fold) in prion infectivity
In the present study, we tested the effectiveness of these two chemical disinfectants using a bioassay and protein misfolding cyclic amplification with beads (PMCAb), which has proven to be a sensitive method to detect even low levels of prion infectivity [7,8,9]

Summary

Introduction

Chronic wasting disease (CWD) is a prion disease affecting cervids, which is recognized in North America, Canada, Republic of Korea, Norway, and Finland [1,2,3]. Using protein misfolding cyclic amplification with beads and a bioassay with TgElk mice, we compared the effects of these disinfectants in CWD-contaminated soil for 1 or 16 h to those of controls of known infectious titres. Our results suggest that 2 N NaOH in a 1/5 farm soil volume provides a large decrease (>102-fold) in prion infectivity.

Results

Conclusion