Abstract
We describe the resolution of the plasma apolipoproteins with molecular weights from 8,800 to greater than 550,000, using a 3.5% sodium dodecyl sulfate-glycerol polyacrylamide slab gel system. The simplicity of this system and the resolution of proteins over a broad range of molecular weights will make it particularly useful in investigations of apolipoprotein composition of plasma lipoproteins.
Highlights
We developed the method of NaDodS04-glycerol polyacrylamidegel electrophoresis for the resolution of apolipoproteins with molecular weights between 6,000 and 80,000 [5]
We report the application of this system to the separation of apolipoproteins with molecular weights from 8,000 to greater than 550,000
ApoB-100, apoE, apoC-111, and apoC-I1 were identified in very low density lipoproteins (VLDL) (Fig. 1, lane 2)
Summary
Electrophoresis grade acrylamide, N,N-methylenebisacrylamide, N,N,N ,N-tetramethylethylenediamine(TEMED), NaDodS04, dithiothreitol (M'T),and ammonium persulfate were obtained from Bio-Rad Laboratories.
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