Sodium-channel and membrane current characteristics of the procerebral neurons of Helix pomatia

Abstract

Event Abstract Back to Event Sodium-channel and membrane current characteristics of the procerebral neurons of Helix pomatia Zsolt Pirger1*, Tibor Kiss1, Izabella Battonyai1 and Károly Elekes1 1 Hungarian Academy of Sciences, Balaton Limnological Research Institute, Hungary Terrestrial snails possess a highly developed olfactory system. Anatomical and physiological data indicate a key role of the procerebrum located in the cerebral ganglion in olfactory processing. The procerebrum of Helix pomatia is comprised of about 50.000 small size (5-8 μm) neurons and this cell population appears to be homogenous. Since there is little information on the membrane compartmentalization and the electrical properties of these procerebral (globuli) cells, in this study we examined, on the one hand, the distribution of different Na-channel subtypes, and on the other hand, the different membrane current characteristics. Applying anti-Nav1.9, anti-Nav1.8 and anti-Nav1.7 antibodies the localization of Na-channel immunoreactive neurns was mapped. All small diameter (5-8 μm) procerebral neurons were labeled by Nav1.8 antibody, but not with anti-Nav1.9 and anti-Nav1.7. In addition, a small population of 10-15 μm diameter neurons, located partly between the procerebral cell mass and the medullary neuropil, and partly on medial surface of the neuropil, displayed anti-Nav1.9 immunostaining. Overlapping of anti-Nav1.7 and anti-Nav1.9 immunolabeling was also observed. In the neuropil a dense varicose fiber system was labeled with the different antibodies applied. The ionic currents of the procerebral cells were studied by whole cell patch clamp. Time- and voltage dependent inward and outward membrane currents could be recorded after step depolarization of the clamped neurons. Holding the neurons at -60 or -40 mV membrane current and depolarizing them by 5 mV steps to +30 mV, three major conductance types were identified: outward delay rectifier potassium current (IK), high-voltage-gated calcium-current (ICa) and sodium-current (INa). The resting membrane potential of the procerebral neurons examined was found in the range of -42 and -55 mV, measured in physiological solution. Support: OTKA grant, No. K78224. Conference: IBRO International Workshop 2010, Pécs, Hungary, 21 Jan - 23 Jan, 2010. Presentation Type: Poster Presentation Topic: Cellular neuroscience Citation: Pirger Z, Kiss T, Battonyai I and Elekes K (2010). Sodium-channel and membrane current characteristics of the procerebral neurons of Helix pomatia. Front. Neurosci. Conference Abstract: IBRO International Workshop 2010. doi: 10.3389/conf.fnins.2010.10.00117 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 28 Apr 2010; Published Online: 28 Apr 2010. * Correspondence: Zsolt Pirger, Hungarian Academy of Sciences, Balaton Limnological Research Institute, Tihany, Hungary, pirgerzs@tres.blki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Zsolt Pirger Tibor Kiss Izabella Battonyai Károly Elekes Google Zsolt Pirger Tibor Kiss Izabella Battonyai Károly Elekes Google Scholar Zsolt Pirger Tibor Kiss Izabella Battonyai Károly Elekes PubMed Zsolt Pirger Tibor Kiss Izabella Battonyai Károly Elekes Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.