Abstract
Histone acetylation is one of the most important posttranslational modifications that contribute to transcriptional initiation and chromatin remodeling. In the present study, we aimed to investigate the effect of sodium butyrate (NaBu), a natural histone deacetylase inhibitor (HDACi), on the maturation of oocytes, preimplantation embryonic development, and expression of important developmental genes. The results indicated that NaBu decreased the rates of GVBD and the first polar body extrusion (PBE) in vitro in a dose-dependent manner. Meanwhile, NaBu treatment led to an abnormality in the spindle apparatus in oocytes in MI. However, the ratio of phosphor-extracellular signal-regulated kinases (p-ERK)/ERK significantly decreased in oocytes treated with 2.0 mM NaBu for 8 h. Furthermore, NaBu treatment at 2.0 mM improved the quality of embryos and the mRNA expression levels of important developmental genes such as HDAC1, Sox2, and Pou5f1. These data suggest that although a high concentration NaBu will impede the meiosis of oocytes, 2.0 mM NaBu will promote the development of embryos in vitro. Further investigation is needed to clarify the direct/indirect effects of NaBu on the regulation of important developmental genes and their subsequent impacts on full-term development in mammals.
Highlights
In mammals, histone modifications, such as acetylation, phosphorylation and methylation, sumoylation, ADP-ribosylation, and ubiquitination, play crucial roles in the development of oocytes/embryos [1,2,3,4]
NaBu at high doses (1.0, 5.0, and 10.0 mM) inhibited the rates of both germinal vesical breakdown (GVBD) and maturation (P < 0.05). These data suggest that NaBu at high doses reduces the rates of GVBD and maturation of germinal vesicle (GV) oocytes
This study aimed to explore the effects of NaBu on the maturation of GV oocytes, preimplantation embryonic development, and the mRNA expression of pluripotent genes
Summary
Histone modifications, such as acetylation, phosphorylation and methylation, sumoylation, ADP-ribosylation, and ubiquitination, play crucial roles in the development of oocytes/embryos [1,2,3,4]. Multiple histone modifications work in concert to regulate diverse biological functions, such as the cell cycle [5], DNA transcription [6, 7], and embryonic development [8]. Disruption of histone modification patterns can impair chromosome. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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