Socializing in experimental streams: crayfish groups exposed to cues, refuges, and day-night conditions

Abstract

We initiated the use of a replicated, remote-video, artificial stream system to determine the effects of conspecific cue, refuge availability, and day/night conditions on movement, aggression, dominance, downstream preference, and refuge use by the crayfish Procambarus clarkii. In line with previous research, we found that walking on the gravel substrate was greatly increased and refuge use decreased in the dark. Conspecific cue (macerated crayfish filtrate) increased walking, contrary to our prediction but consistent with cue as a signal of food availability or of risk either from cannibalism or from visual predators. Exposure to conspecific cue in the streams resulted in a reduced degree of dominance in subsequent pairwise dominance trials. We developed and implemented a new and broadly applicable way of determining the extent of linearity (consistency of dominance ranks) for incomplete dominance; for our data, this method found a lack of evidence for linear hierarchies in these stream systems. Our results suggest that streams may tend to feature weaker dominance and reduced linearity of social hierarchies, relative to standing-water systems, a hypothesis that requires further testing.