Social isolation disrupts hippocampal neurogenesis in young non-human primates.

Simone M Cinini,Luciene Covolan,Gabriela F Barnabe,Magda A De Medeiros,Luiz E Mello,Patrã­Cia Perez-Mendes,Maria B C Sousa,Nicole Galvã£O-Coelho

doi:10.3389/fnins.2014.00045

Abstract

Social relationships are crucial for the development and maintenance of normal behavior in non-human primates. Animals that are raised in isolation develop abnormal patterns of behavior that persist even when they are later reunited with their parents. In rodents, social isolation is a stressful event and is associated with a decrease in hippocampal neurogenesis but considerably less is known about the effects of social isolation in non-human primates during the transition from adolescence to adulthood. To investigate how social isolation affects young marmosets, these were isolated from other members of the colony for 1 or 3 weeks and evaluated for alterations in their behavior and hippocampal cell proliferation. We found that anxiety-related behaviors like scent-marking and locomotor activity increased after social isolation when compared to baseline levels. In agreement, grooming—an indicative of attenuation of tension—was reduced among isolated marmosets. These results were consistent with increased cortisol levels after 1 and 3 weeks of isolation. After social isolation (1 or 3 weeks), reduced proliferation of neural cells in the subgranular zone of dentate granule cell layer was identified and a smaller proportion of BrdU-positive cells underwent neuronal fate (doublecortin labeling). Our data is consistent with the notion that social deprivation during the transition from adolescence to adulthood leads to stress and produces anxiety-like behaviors that in turn might affect neurogenesis and contribute to the deleterious consequences of prolonged stressful conditions.

Highlights

In the adult hippocampus, progenitor cells in the subgranular zone of the dentate gyrus give rise to new neurons that migrate into the granule cell layer, differentiate into granular neurons, and are capable of functional integration into the hippocampal circuitry (Gould and Gross, 2002; Van Praag et al, 2002; Kee et al, 2007)
We found that anxiety-related behaviors like scent-marking and locomotor activity increased after social isolation when compared to baseline levels
In the present study we investigate the consequences of social isolation in the behavior and hippocampal neurogenesis in these non-human primates

Summary

Introduction

Progenitor cells in the subgranular zone of the dentate gyrus give rise to new neurons that migrate into the granule cell layer, differentiate into granular neurons, and are capable of functional integration into the hippocampal circuitry (Gould and Gross, 2002; Van Praag et al, 2002; Kee et al, 2007). The functional role of hippocampal neurogenesis has not been fully understood until now, but despite the divergent results from different laboratories and models, most data points toward its involvement with specific aspects of learning, conditioning, and spatial information (for review see Balu and Lucki, 2009). Social isolation is a form of stress, which affects some hippocampal-related functions such. As learning and memory and may lead to affective disorders. In the present study we used social isolation of young animals as the stressful event (Laudenslager et al, 1995; Stranahan et al, 2006) in order to characterize behavioral consequences of social isolation during the transition phase from adolescence to adulthood, when the animals are at the peak of dentate neurogenesis, so any disturbance might bear a greater relevance in the onset of future mood disorders

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Discussion

Conclusion