Soğan (Allium cepa L.) Rizosferindeki Fosfat Çözen Bakterilerin İzolasyonu, Karakterisasyonu ve Soğan Gelişimi Üzerine Etkisi

İdris Bektaş,Mustafa Küsek

doi:10.18016/ksutarimdoga.vi.811324

Abstract

In this study 269 phosphate solubilizing bacteria (PSB) strains were isolated from onions rhizosphere. The 15 most efficient PSB were selected. Based on BIOLOG profiling and the LOPAT test system, the strains were identiﬁed as Enterobacter cloacae (7), Yersinia intermedia, Pantoea agglomerans, Bacillus subtilis(2), Bacillus pumilius, Lysinibacillus sphaericus and Bacillus atrophaeus(2). The PSB effect on onion growth was investigated under greenhouse conditions. These PSB increased plant height (11.7 to 38.8%), the number of leaves (3 to 70%), the bulb diameter (6.4 to 64.5%), the average root length (2.6 to 44.7%), the fresh weight of the bulb (2.9 to 34.1%) and the dry weight of the bulb (1.5 to 40.6%) when compared to the negative control. Among these strains the most effective inoculants werethe highest P solubizing E.cloacae EB-14 and E. cloacae OB-169 strains, which significantly increased all parameters compared to the commercial plant activator ISR 2000. These results demonstrated the potential use of these PSB as inoculants for onion growth.

Highlights

Onion (Allium cepa L.), a member of Amaryllidaceae family, is one of the most economically important vegetables in all parts of the world due to its nutritional and medicinal values, including anticancer, anti-cholesterol, anti-inflammatory and antioxidant properties (Nasri et al, 2012)
The 15 bacterial strains were identified as Enterobacter cloacae(7), Bacillus atrophaeus(2), Bacillus subtilis(2), Yersinia intermedia, Bacillus pumilus, Lysinibacillus sphaericus and Pantoea agglomerans by using LOPAT tests, Gram tests and BIOLOG profiling (Table 1)
The phosphate solubilizing bacteria (PSB) bacterial strains isolated from the onion rhizosphere exhibited different pathogenicity results on sensitive Kantartopu onion accessions

Summary

Introduction

Onion (Allium cepa L.), a member of Amaryllidaceae family, is one of the most economically important vegetables in all parts of the world due to its nutritional and medicinal values, including anticancer, anti-cholesterol, anti-inflammatory and antioxidant properties (Nasri et al, 2012). In addition to identification studies, the strains were tested for their ability to promote growth under greenhouse conditions in onion plants. The quantitative measurement of phosphate solubilization was carried out using 15 ml test tubes containing 10 ml of NBRIP growth medium freshly inoculated with 0.1 ml of each strain of PSB.

Results

Conclusion