Abstract

This study was conducted to compare the disease-reducing effects of the six antagonistic bacteria, i.e., Bacillus aerophilus HG33, B. pumilus TG71, B. stratospheric AG621, B. subtilis ST115 and Serratia nematodiphila CT78, against Xanthomonas oryzae pv. oryzae causing rice bacterial leaf blight and to screen for the lowest density of the most effective antagonistic bacterial suspension at which the effects were remained under greenhouse conditions. A standard curve for the densities of the six bacteria was made to prepare bacterial suspensions. Seed soaking with the 107-CFU/mL suspensions of B. aerophilus HG33, B. subtilis ST115 and S. nematodiphila CT78 and soil drenching with those of B. pumilus TG71, B. safensis AG131 and B. stratosphericus AG62 were used in the experiments. Results from the two independent replications of this experiment showed that B. stratosphericus AG62 provided the strongest effects among the six bacteria. Therefore, lower suspension densities of this bacterium were tested including 106, 105 and 104 CFU/mL. Soil drenching with the 106-CFU/mL suspension showed similar effects as those of the 107-CFU/mL suspension at all disease assessment time points (5, 10 and 15 days after inoculation).

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