SNX20AR/MiRNA-301a-3p/SNX20 Axis Associated With Cell Proliferation and Immune Infiltration in Lung Adenocarcinoma.

Yixiao Yuan,Xiaolan Zou,Lin Tang,Lincan Duan,Xiulin Jiang,Juan Wang,Qianqian Liu

doi:10.3389/fmolb.2021.744363

Abstract

Lung cancer is the most common tumor with severe morbidity and high mortality. Increasing evidence has demonstrated that SNX20 plays crucial roles in the progression of human cancer. However, the functions and mechanism of SNX20 in LUAD are still barely known. Here, we employ the TCGA, GEO and CCLE databases to examine the expression of SNX20 in human varies cancer, the results shown that SNX20 is down-regulated in lung Adenocarcinoma, SNX20 level was significantly positive correlated with poor prognosis and lung cancer immune cell infiltration. We found that over-expression of SNX20 significantly restrain NSCLC cell proliferation and migration. Subsequently, we discover a network regulating SNX20 in LUAD, further study found that the decreased of the SNX20 likely caused by DNA hypermethylation. Furthermore, we identified that SNX20AR/miRNA-301a-3p mediated decreased of SNX20 correlated with lung cancer progression and cancer immune infiltration in LUAD. Our findings suggested that ncRNAs play a crucial role in the regulatory network of SNX20. Collectively, our findings demonstrate the suppressor roles of the SNX20AR/miRNA-301a-3p/SNX20 axis in Lung Adenocarcinoma, represent that SNX20 have the potential of as an effective therapeutic target in future.

Highlights

Lung cancer is the leading cause of cancer-related death in the world (Sung et al, 2021)
In order to examine the mRNA of SNX20 expression pattern in multifarious cancer, we employed the TIMER tools to analysis the expression of SNX20, the result shown that SNX20 was low expression in LUAD, LUSC, and PAAD, higher expression was observed in BRCA, CHOL, ESCA, GBM, HNSC, KIRC, and KIRP (Figure 1A)
Leukocyte proliferation, regulation of defense response to virus by virus, myeloid dendritic cell activation, interleukin-4 production, leukocyte activation involved in inflammatory response, lymphocyte activation involved in immune response, response to chemokine, immune response regulating signaling pathway and regulation of leukocyte activation (Figure 7C)

Summary

Introduction

Lung cancer is the leading cause of cancer-related death in the world (Sung et al, 2021). Lung adenocarcinoma is the most common molecular subtype of NSCLC cancer, and LUAD accounts for almost 50 percent of lung cancers. It’s extremely urgent to discerned accurate and sensitive immune-related biomarkers and elucidate the molecular mechanisms participate in LUAD progression. The SNX subgroup mainly including the sorting nexin-20, sorting nexin-21 and the SNX-PXB proteins (Zeng et al, 2002). These proteins has a C-terminal PX-associated B (PXB) domain which is essential for protein interactions

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