Abstract
Soybean seed protein content (SPC) has been decreasing throughout last decades and DNA marker association has shown its usefulness to improve this trait even in soybean breeding programs that focus primarily on soybean yield and seed oil content (SOC). Aiming to elucidate the association of two SNP markers (ss715630650 and ss715636852) to the SPC, a soybean population of 264 F5-derived recombinant inbred lines (RILs) from a bi-parental cross was tested in four environments. Through the single-marker analysis, the additive effect () and the portion of SPC variation due to the SNPs () for single and multi-environment data were assessed, and transgressive RILs for SPC were observed. The estimates revealed the association of both markers to SPC in most of environments. The marker ss715636852 was more frequently associated to SPC, including multi-environment data, and contributed up to = 1.30% for overall SPC, whereas ss715630650 had significant association just in two locations, with contributions of = 0.76% and = 0.74% to overall SPC in Vic1 and Cap1, respectively. The RIL 84-13 was classified as an elite genotype due to its favorable alleles and high SPC means, which reached 53.78% in Cap1, and 46.33% in MET analysis. Thus, these results confirm the usefulness of the SNP marker ss715636852 in a soybean breeding program for SPC.
Highlights
Soybean [Glycine max (L.) Merr.] is a major crop widely cultivated worldwide, and its importance is mainly assigned to its seed protein content (SPC), denoting the relevance of this crop for human and animal nutrition, as well as economy and world food security
In order to compare the environments, the SPC means of 10% superior recombinant inbred lines (RILs) were 52.84, 51.49, 49.39, 48.63, and 47.84% for Cap 1, Cap2, Vic1, Vic2 and MET dada, respectively
Checks were outperformed in an average of 5.40% in all trial scenarios, which is inferior to the results reported by Warrington et al (2015), where F5derived RILs mapping population exceeded the cultivars in a rate of 7.50% for SPC
Summary
Soybean [Glycine max (L.) Merr.] is a major crop widely cultivated worldwide, and its importance is mainly assigned to its seed protein content (SPC), denoting the relevance of this crop for human and animal nutrition, as well as economy and world food security. SPC is quantitatively inherited and correlates negatively with most of the main traits taken into account in a soybean-breeding program (Kwon & Torrie, 1964). By using the marker information within or in proximity to important protein-related QTLs (Quantitative Trait Loci), strategies involving the validation of molecular markers in structured populations have been combined with traditional breeding methods in order to deliver more rapid genetic gains in many soybean traits (Jun et al, 2008)
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