SNF472 inhibits the progression of vitamin D induced cardiovascular calcification in rats

Abstract

A total of 87 male Sprague Dawley rats were used in this study. Of these 45 were used to evaluate the dose-response of vitamin D in producing CVC. Calcification was induced through daily (day 1 to day 3) s.c. administration of 3, 10, 30, 100 or 300 kIU/kg of vitamin D3. A subset of three animals from each group was sacrificed and evaluated on days 3, 4 and 5 (Figure 1A). The remaining 42 animals were administered 100 kIU/kg vitamin D and divided into five groups, each group receiving daily s.c. vehicle or SNF472 for up to five days (Figure 1B). G1 received daily vehicle; three animals were sacrificed every day from day 1 to day 4 to evaluate CVC progression and 6 animals were sacrificed on day 5. Four more groups of six animals (G2, G3, G4, G5) were dosed with SNF472 at 100 mg/kg, starting on day 1, 2, 3 or 4, respectively, and sacrificed on day 5. AIM