SncRiboTag-Seq: Cell-type-specific RiboTag-Seq for cells in low abundance in mouse brain tissue

Abstract

SummaryThe mammalian brain has over 10,000 types of neurons. Therefore, studying gene regulation in the brain requires effective strategies for targeting specific cell types, especially those in low abundance. Cell isolation may alter gene expression and is disruptive to mature neurons with extensive processes. This protocol describes cell-type-specific expression of tagged ribosome and the use of ribosome tagging followed by RNA-seq to identify translatome of low number and sparse cells in mouse brains without disruptive cell isolation.For complete details on the use and execution of this protocol, please refer to Gao et al. (2020)