Abstract
SNARE proteins mediate membrane fusion between synaptic vesicles and the plasma membrane. A minimized peptide SNARE model system with reduced complexity was introduced combining the native SNARE transmembrane (TMD) and linker domains with artificial coiled-coil forming peptides. Specific membrane fusion initiated by coiled-coil recognition was shown by lipid and content mixing vesicle assays.
Highlights
SNARE proteins mediate membrane fusion between synaptic vesicles and the plasma membrane
Neuronal exocytosis is catalyzed by the SNARE proteins SNAP-25, VAMP2, and syntaxin-1A, with the two latter each possessing a single transmembrane domain (TMD) at the C-terminus (Fig. 1a).[1,5]
We have recently introduced a new SNARE-mimicking model using the native TMDs linked to peptide nucleic acid (PNA) recognition motifs and obtained efficient fusion that proceeds via hemifusion as intermediate.[16]
Summary
SNARE proteins mediate membrane fusion between synaptic vesicles and the plasma membrane. A minimized peptide SNARE model system with reduced complexity was introduced combining the native SNARE transmembrane (TMD) and linker domains with artificial coiled-coil forming peptides.
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