Abstract
Five non-neurotoxic polypeptides, designated V II1–V II4 and V 5, have been isolated from the venom of Naja mossambica mossambica by column chromatographic techniques. Whole venom was fractionated on Sephadex G-50 and the major fraction, thus obtained, was re-chromatographed on CM-cellulose and Amberlite CG-50 to yield the polypeptides in pure form. They consist of 60 amino acid residues each and have molecular weights of 6820, 6792, 6888, 6712 and 6991, respectively. Immunochemically, polypeptides V II1–V II4 could be identified as cardiotoxins. The position with respect to Polypeptide V 5 was unclear and did not allow a definite assignment. This is the first time that the isolation of more than two cardiotoxins from the venom of the same specie has been described. Polypeptide V II1 was found to have the same amino acid composition as toxin γ, a cardiotoxin that has been isolated from the venom of Naja mossambica pallida.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Protein Structure
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