Abstract
Due to its pivotal role in many physiological processes and calcium-depleting diseases like osteoporosis, accurate quantification of the concentration of calcium in blood serum is imperative in monitoring multifarious facets of human health and disease. Established laboratory-based protocols for serum calcium level detection are expensive, resource-intensive and functionally dependent on skilled technicians. Circumventing these constraints, here we innovate a novel adaptation of the Bradford’s assay as a decisive pre-processing step of a highly specific diagnostic test for serum calcium level detection. This ensures the binding of protein molecules to Coomassie dye under acidic conditions with a resulting alteration in its colour so as to quench out the possibilities of unwarranted side reactions with excess proteins abundantly present in patient samples. This specific adaptation renders the test to be implemented on a simple paper strip without deploying controlled laboratory-based procedure, obviating any adverse interference in the subsequent reaction of calcium in the serum with Arsenazo III, a metallochromic dye used for the final colorimetric detection step. The method is affordable, user-friendly and can be deployed by minimally trained personnel at the point of use in extreme harsh environments. By mapping the resulting colorimetric information quantitatively with the serum concentration level from a panel of training dataset pre-standardized via established laboratory-based gold standard examination, a simple smartphone-based readout system may be developed, bearing the potential of replacing the currently existing expensive, time-consuming and environmentally-restrictive diagnostic solutions.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.