Abstract
We address in this paper a powerful point-of-care platform to conduct the Bradford assay. Our method was based on smartphone for colorimetric quantification of total protein in human blood plasma, presenting low cost, simplicity, portability, autonomy and ability for remote transmission of the data. Other advantage concerns the high number of smartphone's users worldwide. This feature contributes for the application of the method by non-specialist people. The interferences from external light were successfully solved by illuminating the samples with a straightforward negatoscope. Our method generated a satisfactory exactness, with accuracy percentages ranging from 87.2 to 99.1%.
Highlights
Bradford assay is one of the most usual methods applied for determining protein in solution.[1,2,3,4] This method was proposed by Marion M
Analytical curves for Bovine serum albumin (BSA) standards and some parameters (R2, analytical sensitivity, S, and LOD) obtained by the commercial spectrophotometer and smartphone are depicted in Figures 3a and 3d, respectively
The spectrophotometer generated best linearity (R2 > 0.99) and detectability compared with smartphone
Summary
Bradford assay is one of the most usual methods applied for determining protein in solution.[1,2,3,4] This method was proposed by Marion M. We address a POC platform for performing the Bradford assay using smartphone-based optical detection. Concerning the step of application to the samples, the concentrations of protein (C) were calculated from the data of the regression straight equation related to the analytical curve, namely: linear coefficient (a) and analytical sensitivity (angular coefficient, S).
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