Smart plasmonic Ag/Ag2O/ZnO nanocomposite with promising photothermal and photodynamic antibacterial activity under 600 nm visible light illumination

Abstract

• Smart plasmonic Ag/Ag 2 O/ZnO nanocomposite was fabricated. • Excellent photothermal conversion property under 600 nm light illuminating. • Ag serves as a plasmonic material enhancing the light absorption in the visible region. • Promising antibacterial activity upon 600 nm illumination against Enterococcus faecalis . Photoactivated antibacterial activity is a very promising strategy to fight bacteria without inducing bacterial resistance. This contribution combines the photodynamic and photothermal properties along with plasmonic effect in a single nanocomposite to effectively kill bacteria. Plasmonic Ag/p-Ag 2 O/n-ZnO heterojunction nanocomposites with well-defined morphologies are synthesized via solution combustion using glycine and thyme extract as fuels. Ag/Ag 2 O/ZnO shows excellent photothermal conversion behavior under 600 nm light illuminating. ESR analysis confirms the production of reactive species (ROS) under 600 nm. Ag acts as plasmonic material enhancing the light absorption in the visible region. FT-IR confirms the presence of thyme-related function groups on the surface of the prepared nanocomposite which also contribute to enhancing the antibacterial activity. Ag/Ag 2 O/ZnO shows promising antibacterial activity under 600 nm illumination against Enterococcus faecalis . Moreover, the nanocomposite shows excellent antibacterial activity against C. albicans and S. epidermidis in the dark. The efficient bacterial killing under 600 nm light illumination is attributed to the synergetic effects among photothermal, photodynamic, and plasmonic effects. The antibacterial activity in the dark is due to the intrinsic antibacterial activity of Ag, ZnO, and Ag 2 O along with thyme plant extract. Cytotoxicity of Ag/Ag 2 O/ZnO on Huh-7 human liver cancer cells line is also investigated and the IC 50 values calculated are 112.9 and 313 μg/mL for the Ag/Ag 2 O/ZnO samples prepared using 3ml (3AgZn) and 7 ml AgNO 3 (7AgZn) respectively.