Abstract
Herein, a smart porous material, Cu-hemin metal-organic-frameworks (Cu-hemin MOFs), was synthesized via assembling of Cu2+ with hemin to load glucose oxidase (GOD) for electrochemical glucose biosensing for the first time. The formation of the Cu-hemin MOFs was verified by scanning electron microscopy, X-ray powder diffraction, Fourier transform infrared spectroscopy, N2 adsorption/desorption isotherms, UV-vis absorption spectroscopy, fluorescence spectroscopy, thermal analysis and electrochemical techniques. The results indicated that the Cu-hemin MOFs showed a ball-flower-like hollow cage structure with a large specific surface area and a large number of mesopores. A large number of GOD molecules could be successfully loaded in the pores of Cu-hemin MOFs to keep their bioactivity just like in a solution. The GOD/Cu-hemin MOFs exhibited both good performance toward oxygen reduction reaction via Cu-hemin MOFs and catalytic oxidation of glucose via GOD, superior to other GOD/MOFs and GOD/nanomaterials. Accordingly, the performance of GOD/Cu-hemin MOFs-based electrochemical glucose sensor was enhanced greatly, showing a wide linear range from 9.10 μM to 36.0 mM and a low detection limit of 2.73 μM. Moreover, the sensor showed satisfactory results in detection of glucose in human serum. This work provides a practical design of new electrochemical sensing platform based on MOFs and biomolecules.
Highlights
As an important research area of analytical chemistry[1,2,3,4,5], electrochemical glucose biosensors have received significant attention over past few years because of their low cost, quick response, simple preparation and wide applications in biomedical, clinical research, food production, ecology and even textile industry[6,7,8,9]
The glucose oxidase (GOD) molecules were incorporated into Cu-hemin Metal-organic frameworks (MOFs) by utilizing the associated pores of MOFs, which effectively avoided the aggregation of enzyme on the surface of electrode
It could be seen that the Cu-hemin MOFs showed a big hollow cage and a large number of holes in the flower-like ball, which could provide a large specific surface area and enhance the mass transfer (Fig. 1B)
Summary
As an important research area of analytical chemistry[1,2,3,4,5], electrochemical glucose biosensors have received significant attention over past few years because of their low cost, quick response, simple preparation and wide applications in biomedical, clinical research, food production, ecology and even textile industry[6,7,8,9]. The novel Cu-hemin MOFs showed a 3D ball-flower-like nanostructure and was hollow inside, which could be used to load a large number of GOD molecules.
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