Smart multifaceted potential microbial inoculant isolated from rhizospheric soils of Bergenia ciliata and possible role in developing green biosynthesized nanoparticles

Abstract

Nanotechnology employs miniscule yet potential nanoparticles and has immense applications in various fields of scientific research. This has revolutionized technological advancements in multiple spheres and recently touched the field of plant biology and sustainable agriculture. Beneficial rhizobacteria isolated from plants may prove efficacious as they are able to impart plant growth. From the medicinal plant Bergenia ciliata, one isolate BC-I-15 was characterized in this study and displayed growth promoting traits. 16s rDNA sequencing and molecular characterization confirmed the isolate as Pseudomonas sp. (NCBI accession number- OQ891764) which showed similarity with other Pseudomonads in phylogenetic tree. The isolate exhibited oxidative stress tolerance assay, nitrogen fixing ability, IAA, calcite and phosphate solubilization etc. But such microbial bioinoculants suffers from few limitations and can be enriched when their nanoparticle counterparts are developed for efficient function. The citation network analysis showed good and encouraging relationships between terms like ‘Nanoparticles, Microbes, Sustainable agriculture’. So, when such beneficial microbes in bulk form, or biofilm and exopolysaccharides (EPS) secreting, secondary metabolites etc. parts thereof are used as starting material for nanoparticle formation, then such nanoparticles possess intrinsic and enriched capacity to impart stress tolerance to plants and impart qualities in sustainable agriculture. The results of the present study, further aids in the efficient utilization of the selected beneficial BC-I-15 rhizobacteria and their future exploration in developing biogenic nanoparticles for active agents in sustainable agriculture.