Small-Molecule Sequestration Using Aptamer-Functionalized Membranes

Abstract

Sequestration of small molecules from aqueous solutions poses a significant and important challenge in environmental science and human health. Current methods focus on broadly sequestering all small molecules but are unable to address specific small molecules of interest. Additionally, these procedures require large amounts of resources, such as electricity and pressure. We propose to address this challenge through the use of DNA aptamer-functionalized ultrafiltration membranes. To demonstrate this approach, we developed an aptamer-functionalized membrane that sequesters and removes the small-molecule contaminant bisphenol A (BPA) from water. We show that BPA is depleted and that the membranes can be regenerated for multiple uses, which can allow for recovery of the small molecule when desired. Aptamers can be selected for a wide variety of target small molecules, making this approach highly generalizable beyond our initial demonstration. Together, this research offers a promising solution to improving the efficacy of small molecule removal and recovery from aqueous matrices.