Abstract
The construction and the use of a simple and unexpensive apparatus for the ionophoretic separation of amino acids is described. By reducing the size of the filterpaper and by employing a very small amount of a volatile buffer (formic acid-acetic acid) the requirement of electric potential is much reduced, and at 650–700 V a good resolution of urinary and probably other naturally occurring amino acids is obtained. In normal urines 11–17 ninhydrine positive fractions were found. No desalting is required and the run is completed in 60–80 min.
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