Abstract

Small spherical virus particles were found in the phloem cells of broad bean plants infected with milk-vetch dwarf virus. The virus particles appear in the degenerated cytoplasm or in the vacuoles, forming amorphous, sometimes crystalline, aggregates. The virus particles could be partially purified by chloroform-butanol clarification followed by differential centrifugation. Their particle size is approximately 26nm in negatively stained preparations.

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