Abstract
Respiratory syncytial virus (RSV) or measles virus (MeV) infection modifies host responses through small non-coding RNA (sncRNA) expression. We show that RSV or MeV infection of neuronal cells induces sncRNAs including various microRNAs and transfer RNA fragments (tRFs). We show that these tRFs originate from select tRNAs (GCC and CAC for glycine, CTT and AAC for Valine, and CCC and TTT for Lysine). Some of the tRNAs are rarely used by RSV or MeV as indicated by relative synonymous codon usage indices suggesting selective cleavage of the tRNAs occurs in infected neuronal cells. The data implies that differentially expressed sncRNAs may regulate host gene expression via multiple mechanisms in neuronal cells.
Highlights
Small non-coding RNAs are
We identified that (1) miR-10b-5p was expressed in mock-treated cells but showed reduced expression in Respiratory syncytial virus (RSV)- or measles virus (MeV)-infected cells, (2) p-miR-247 and miR-375 had robust expression in RSV- or MeV-infected cells, (3) miRs had no/low expression in mock-treated cells, but were increased by RSV or MeV infection, and (4) miRs were not expressed in mock-infected cells at 12 hpi but showed robust copy numbers at 24 hpi
These findings show that RSV or MeV infection induces distinct miR expression in SHS cells that is virus-type and time-point specific
Summary
Small non-coding RNAs (sncRNAs) are
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