Abstract
Listeria monocytogenes is a Gram-positive foodborne pathogen and the causative agent of listeriosis. L. monocytogenes lapB gene encodes a cell wall surface anchor protein, and mutation of this gene causes Listeria attenuation in mice. In this work, the potential role of Listeria LapB protein in catfish fillet attachment was investigated. To achieve this, boron-based small molecules designed to interfere with the active site of the L. monocytogenes LapB protein were developed, and their ability to prevent L. monocytogenes attachment to fish fillet was tested. Results indicated that seven out of nine different small molecules were effective in reducing the Listeria attachment to catfish fillets. Of these, three small molecules (SM3, SM5, and SM7) were highly effective in blocking Listeria attachment to catfish fillets. This study suggests an alternative strategy for reduction of L. monocytogenes contamination in fresh and frozen fish products.
Highlights
Listeria monocytogenes is a Gram-positive foodborne pathogen causing listeriosis, which has a high mortality rate [1]
LapB protein is involved in L. monocytogenes pathogenesis and potentially mediates attachment to host cell surfaces
To target LapB protein, nine different compounds were synthesized with different pharmacophore groups
Summary
Listeria monocytogenes is a Gram-positive foodborne pathogen causing listeriosis, which has a high mortality rate [1]. L. monocytogenes was discovered in the 1930s as a pathogen of animals and humans. It was initially identified as the cause of miscarriage in early pregnancy, stillbirth, and septicemia after an uneventful birth. L. monocytogenes shows elevated heat resistance, growth at refrigeration temperatures, tolerance to reduced pH, and growth in the presence of over 5% sodium chloride; it is a substantial foodborne pathogen in ready-to-eat aquaculture products [2,3,4]. Numerous studies have shown that over a quarter of frozen seafood was contaminated with L. monocytogenes [5, 6]. Serotypes 4b, 1/2a, 1/2b, and 1/2c are considered highest risk, while serotypes 3a, 3b, 3c, 4a, 4c, 4d, and 4e are considered low-risk for listeriosis [11]
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